Protein Structure from Scientific Research

Top: in the ADP·Pi state, F360 from the trans-acting subunit samples two side-chain rotamer states, one of which contacts helix α 407–423 of the active subunit. Bottom: crosslinking of C360 to this helix at C413, but not the mutations alone, abolishes ATPase activity of D1. Δ designates a cysteine-free p97 variant. Data are presented as mean values. Error bars represent s.d. for n = 4 biologically independent replicates. ATPase rates were determined in n = 3 or 4 replicates, as indicated by the corresponding data points.

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