Line Plot from Scientific Research

Left and middle: magic-angle spinning 1 H → 31 P cross-polarization NMR spectra of p97-bound nucleotide in the presence of ATP (top) and ADP (bottom). The p97-ND1L hexamer (residues 1–480) is ATPase active 6 and contains only signals from D1; the corresponding spectra of full-length p97 are shown in lighter hues. Right: the observation of multiple weaker signals (orange fit) is ascribed to phosphate ions in chemically distinct environments. These signals must derive from the cleaved γ-phosphate of ATP locked in the D1 active site, because thio-substitution at this position in ATP results in a strong downfield shift 11 .

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