Protein Structure from Scientific Research

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Single-particle cryo-EM reconstruction of p97 in the ADP·Pi state reveals a symmetric hexamer with NTD domains in the ‘down’ position. For comparison, cryo-EM maps of ATPγS- and ADP-bound p97 are shown (EMDB 3298 and 3299 ) 5 . The colouring reflects the bound nucleotides shown underneath. Details on reconstruction are provided in Supplementary Figs. 1 – 3 .
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