Scatter heat map of a red-green projection of data for a simple mixture of EGFP + AB12, FLuc + AB6 and AB10 (no target). Points are coloured according to the local relative scatter-plot density. The inset shows the scatter plot of signals from unbound FRETfluors of all types (grey) and bound EGFP + AB12 (orange) and FLuc + AB6 (red). Each point in the main panel and the inset represents 1,000 photons, with ‘bound’ and ‘unbound’ assignments based on localization trajectories and red-green brightness taken from the corresponding level with duration of >100 ms; N = 6,137 total points.
