Expression profiles of major genes associated with the activation or differentiation of immunoregulation, cellular processes, signal transduction and inflammation in lymph nodes at 7, 14 and 28 days after primary immunization and 21 days after booster immunization. The colour bars represent the log 2 (fold change) compared with the value in the LNP group. The redder the colour, the more obviously the gene transcription level is upregulated. The relative expression levels of inflammatory cytokines in tissue were normalized to their levels in the blank control group (LNP) by using the comparative Ct (ΔΔCt) method. The data are shown as the means ± s.d. from three independent experiments ( n = 3). Significant differences were determined using one-way ANOVA with Tukey’s post hoc test.
