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Triple labelling of CTC-445.2d. In the first step, Oa AEP1 was used to attach a C-terminal alkyne click handle via incorporation of propargylamine. Next, sortase A was used to label the protein N terminus with TAMRA-LPET. Finally, the KL-tag was acylated with biotin-RN in a second Oa AEP1-catalysed step. For all panels, the spectra shown are reconstructed ESI-MS spectra. The peptide label:protein ratios were calculated based on relative peak heights and are shown adjacent to the spectra.

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