Cryo-EM map and model of the SOI–NB complex structure at 2.05 A resolution. Each subunit of the SOI trimer (blue, light blue, cyan) is bound to a nanobody (NB, orange or yellow) via the periplasmic loops. Densities for the MSP1D1 nanodisc and for the disordered regions of SOI (C terminus and N-terminal 6xHis-tag, are coloured in light grey.