Competition between bis- p NPP and cAMP hydrolysis (left) shown in a Lineweaver–Burke plot (right). Lines connect kinetics for a single cAMP concentration, with higher cAMP concentrations shown in darkening hues of green. The shared y intercept indicates competitive inhibition with K i = 70 ± 8 µM cAMP. Michaelis–Menten kinetics were assayed for bis( p -nitrophenyl) phosphate hydrolysis by 100 µM protein with 200 µM MnCl 2 in increasing cAMP concentrations.
