The number of mutation steps from the given wild-type variant hGLP-1 is indicated. Designs P2 and P3 are dual co-agonists, with potency seven- and tenfold higher than human glucagon at hGCGR, and simultaneously P3 has 3.5-fold higher potency than hGLP-1 at hGLP-1R. For each peptide, the colour of the marker corresponds to the designated activity group: yellow, dual agonist; green, GCGR-selective; blue, GLP-1R-selective.
