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RT–qPCR in U2OS cells stably expressing the MRTFA-GFP and (GS) 4 -MRTFA-GFP, 4 h after serum stimulation ( N = 7 independent experiments). Bars show mean s.d. Significance levels for the two-tailed paired t -test after log transformation: * P < 0.01; ** P < 0.001; SRF, P = 7.16 10 −3 , MYL9, P = 0.04.
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