Dot blot intensity for each of the Nups interacting with Ig27 WT , normalized by the control experiment. Each data point corresponds to the average intensity of three dots per membrane ( N =5 independent dot blot experiments). Significance levels for two-tailed t -test: NS, P > 0.05; ** P < 0.01; Nup153 versus Nup214, P = 6.94 × 10 −3 ; Nup214 versus Nup98, P = 0.76; Nup153 versus Nup98, P = 1.42 × 10 −3 . Boxes span from the first to the third quartile, and the horizontal line indicates the median.
