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Schematic showing that the addition of a mechanically labile peptide tag next to the NLS substantially increases the kinetics of the nuclear import of protein cargos across the NPC. Significance levels for the Mann–Whitney non-parametric test: NS, P > 0.05; * P < 0.05; *** P < 0.001; **** P < 0.0001. All points and bar plots indicate mean ± s.e.m.

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