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Total unfolding rate r N of an Ig27 WT polyprotein as a function of N , calculated as r N = 1/ t N . The resulting unfolding kinetics can be reproduced by a stochastic model where r N = r U /Σ(1/ n ), with n = 1, …, N (red dashed line) and with r U = 1.15 s −1 . For comparison, a sequential unfolding process would exhibit a steeper dependence on N (black dotted line , r N = r U / N ).
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