The number of visible turns as a function of time, before, during and after optogenetic generation of a myosin cluster (left: experiment, for the doublet shown in f ; right: simulations). Scale bars: 5 m. Time in hh:mm in a and d or mm:ss in f . Grey lines in b , c , e , h : Individual trajectories. Error bars show the 95% confidence interval of the mean. N = 3 biological repeats. Statistical tests: In b , the average at t = 75 min is compared with the average for t 0 min; In c , the average height at t = 90 min is compared with the average height for t 0 min. P values are (3.1 1.4) 10 5 in e , (2.2 0.1) 10 3 in g and (5.38 0.14) 10 3 in h , and *** P < 10 4 otherwise (see Supplementary Section 10 for details).
