Predicted affinities for top PT-derived peptides predicted to bind to alleles with those containing a threonine at position 233 of HLA-DRB1 (‘DRB1-233Thr’, n = 11) compared to those with an arginine (‘DRB1-233Arg’, n = 101) calculated from the Immune Epitope Database (IEDB), with a difference tested using a two-tailed Mann–Whitney test. In the box plot, the center line represents the median, the box limits denote the upper and lower quartiles, and the whiskers are 1.5 times interquartile range. ** P < 0.01; NS, not significant.