Workflow from Scientific Research

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In SAMOSA-Tag, nuclei were methylated using the non-specific EcoGII m 6 dAase and tagmented in situ with hairpin-loaded transposomes. DNA was purified, gap-repaired and sequenced, resulting in molecules where the ends resulted from Tn5 transposition, the m 6 dA marks represented fiber accessibility and computationally defined unmethylated footprints captured proteinDNA interactions.
#Workflow#Illustration#SAMOSA-Tag#Methylation#EcoGII m6dAase#Transposomes#DNA#Tn5 transposition#Protein-DNA interactions
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