Workflow from Scientific Research

Open access visualization of Workflow, Illustration, SAMOSA-Tag, Methylation, EcoGII m6dAase
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In SAMOSA-Tag, nuclei were methylated using the non-specific EcoGII m 6 dAase and tagmented in situ with hairpin-loaded transposomes. DNA was purified, gap-repaired and sequenced, resulting in molecules where the ends resulted from Tn5 transposition, the m 6 dA marks represented fiber accessibility and computationally defined unmethylated footprints captured proteinDNA interactions.

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