In SAMOSA-Tag, nuclei were methylated using the non-specific EcoGII m 6 dAase and tagmented in situ with hairpin-loaded transposomes. DNA was purified, gap-repaired and sequenced, resulting in molecules where the ends resulted from Tn5 transposition, the m 6 dA marks represented fiber accessibility and computationally defined unmethylated footprints captured proteinDNA interactions.
