Bar plots showing expression of the indicated genes in wild-type, Mll2 CM/CM and Mll2 CM/CM + Prdm9 scFV ESCs, in which H3K4me3 has been programmed back to a repressed promoter that previously lost H3K4me3. Shown is the mean of three biological replicates assayed by qPCR with reverse transcription (RTqPCR). Error bars represent s.d., and significance of rescue was calculated by two-tailed unpaired t-test.