Programming of a specific chromatin modification (left) and transcriptional responses in single cells (right) for H3K4me3. Left: histogram showing relative (rel.) enrichment of the indicated chromatin modification after targeting control GFP scFV (gray bar), wild-type CD scFV (red bar) or catalytically inactive mut-CD scFV (blue bar) for 7 d. Displayed is the mean of at least two independent quantitations by CUT&RUNqPCR or ChIPqPCR. Error bars represent s.d. Rep, reporter. Right: dot plot showing log 10 (mCherry expression) in response to epigenetic editing of the indicated chromatin mark. n = 250 individual cells; bars denote geometric mean of the population; gray shading indicates control geometric mean. Reading was performed for four independent experiments. P values were calculated by one-way ANOVA with Tukeys multipletest correction.
