Relative abundance of the indicated histone modification (H2AK119ub) across the Hbb-y locus after epigenetic programming with a specific CD scFV (Ring1b; red line) or control GFP scFV (gray line), assayed by CUT&RUNqPCR. Mean enrichment across a ~14-kb region centered on the gRNA-binding site is shown for editing in biological triplicates as well as for endogenous positive (Pos1 and Pos2) and negative (Neg1 and Neg2) loci for each mark. NS, not significant.
