Gene family expansion and contraction patterns estimated by CAFE using Orthofinder-identified OGs and the time-calibrated phylogeny of Fig. 1g. Key nodes are indicated on the tree and significant expansions and contractions are shown. The circles are proportional to expanded/contracted OGs; the numbers next to the circles indicate the numbers of expanded (orange) and contracted (dark gray) OGs. Z. cir., Zygnema circumcarinatum; Z. cyl., Zygnema cf. cylindricum. Icons indicate body plans: parenchymatous (box of tissue), filamentous (chain of cells), unicellular (single round cell) and sarcinoid/colonial (two round cells).