Microscopy from Scientific Research

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Chromosome counting on light micrographs of SAG 698-1b fixed and stained with acetocarmine at prophase (0.5 months old); count was also performed in metaphase and telophase (Supplementary Fig. 1 ). The green dots represent the 20 chromosomes that were counted after rendering a stack of ~100 images. Scale bar, 10 m. See Supplementary Fig. 1 for the original images. A minimum of ten cells each from three independent cell cultures were analyzed.
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