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Western blot analysis of whole-cell lysates collected from EZH2 knockout and rescued K562 cell lines, as indicated. The lentivirus EZH2 rescue vector is indicated, with an empty vector used as a control (Ctrl). The lentiviral gRNA vector is indicated, either if EZH2 gRNA (+) or the AVVS gRNA control vector (-) is used. Immunoblotting antibodies are indicated on the left. Shown are from two independent replicates that were carried out on two different days, with an additional four independent replicates shown in Supplementary Fig. 2a.

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