Top, CRISPRi tiling screen results for each target gene (rows) in T conv cells (top) and T reg cells (bottom). Gray regions indicate costimulatory gene bodies. Stimulation-responsive and T reg -dominant CiREs are manually labeled. CRISPRi tiling results are plotted as in Fig. 2 . Middle, ATAC-seq profiles in T conv cells (top) and T reg cells (bottom). Bottom, one-dimensional (1D; top) and two-dimensional (2D; bottom) CTCF-bound genomic contacts identified by ChIA–PET in human CD4 + T cells ( Methods ). Dashed outlines indicate CiREs colocalizing with CTCF ChIA–PET peaks.
