Heatmap from Scientific Research

A 4C-seq plot anchored on the stimulation-responsive CiRE for T conv cells restimulated for 6 h from one donor (biological replicate of Extended Data Fig. 9d ) subjected to CRISPRi-mediated CTCF-2 disruption (green) or a nontargeting control (black). The positions for the stimulation-responsive CiRE probe (serving as the 4C viewpoint) and the CTCF-2 boundary are indicated by arrowheads. Dashed box regions indicate CD28 , CTLA4 and ICOS gene bodies. CRISPRi tiling screen results in T conv cells are plotted in one dimension (top). Hi-C from CD4 + T cells is plotted (bottom).

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