Normalized 4C signal intensity comparing nontargeting control (NTC) and CRISPRi-mediated CTCF-2 disruption for the CD28 ( c ), CTLA4 ( d ) and ICOS ( e ) gene bodies, with results of two-sided t -tests with Bonferroni correction. Each point represents the log-transformed 4C-seq signal intensity of each captured genomic region in the respective gene bodies. Results from one donor with accompanying biological replicate data in Extended Data Fig. 9e–g . P adj , adjusted P value.
