Fold change of CTLA4 median fluorescence intensity in primary T reg cells (n = 2 donors) restimulated for 6 h under arrayed CRISPRi validation of sgRNA species targeting the stimulation-responsive CiRE (n = 4), the T reg -dominant CiRE (n = 4) or the CTLA4 TSS (n = 5) relative to nontargeting controls (n = 7).
