Gene bodies across the TAD designated in b atop CRISPRi tiling screen results for each target gene (rows) in T conv cells (top) and T reg cells (bottom) from two human donors. Each point signifies the genomic position ( x axis) and −log 10 -transformed unadjusted P value ( y axis) of each sgRNA using the Wald test with Benjamini–Hochberg correction. Blue indicates sgRNA species significantly enriched (adjusted P < 0.05) in the lowest 20% of target protein-expressing cells, and gold indicates sgRNA species significantly enriched (adjusted P < 0.05) in the highest 20% of target protein-expressing cells. Flow cytometry histograms of target protein expression for each screen are included in the right margin, including the gated populations isolated for sgRNA quantification. The window labeled ‘ CTLA4 regulatory region’ is expanded in Fig. 2 . Restim, restimulation.
