Illustration from Scientific Research

Pedigrees of additional mouse families. Black denotes treated groups. Unshaded siblings comprise control groups. For the PE2* family (left), treated embryos were microinjected with PE2* components (PEmax mRNA, Chd2 + 5 G > A pegRNA) at the two-cell stage, whereas control embryos were microinjected with pegRNA only. For the mMLH1dn family (right), treated embryos were microinjected with mMLH1dn mRNA and the Chd2 + 5 G > A pegRNA (but no editor) at the two-cell stage, whereas control embryos were microinjected with pegRNA only. One control embryo (not indicated) was omitted from analysis for this family after sequencing failed quality control (Methods). Percentages indicate precise edit efficiency in treated embryos at E12.5.

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