Pedigree of PE4 family (top). Black indicates the treated group of select progeny microinjected at the two-cell stage with PE4 components (PE2 editor mRNA, Chd2 + 5 G > A pegRNA, mMLH1dn mRNA). Unshaded family members indicate mice/embryos treated as the control group, including sibling progeny microinjected at the two-cell stage with PE2 editor mRNA only. Percentages indicate precise edit efficiency at E12.5 as determined by WGS. Plot (bottom) compares editing frequencies in treated embryos across sequencing methods (target versus whole-genome sequencing). Superscripts denote individual progeny from PE4 family. Dashed line represents x = y.
