Cell number (above) and EpiTrace age (below) of T ex cells, separated by treatment response (R: responder; NR: non-responder) and T cell phenotypic maturity (Immature/Interim/Mature). Sample numbers of independent biological cells: n = 322 (NR group, Immature cell, Pre-PD1); 596 (NR group, Immature cell, Post-PD1); 77 (NR group, Interim cell, Pre-PD1); 108 (NR group, Interim cell, Post-PD1); 97 (NR group, Mature cell, Pre-PD1); 14 (NR group, Mature cell, Post-PD1); 77 (R group, Immature cell, Pre-PD1); 237 (R group, Immature cell, Post-PD1); 222 (R group, Interim cell, Pre-PD1); 923 (R group, Interim cell, Post-PD1); 378 (R group, Mature cell, Pre-PD1); and 2,452 (R group, Mature cell, Post-PD1).