Single-cell age estimated with EpiTrace from f . The induced cultures were either subjected to the full induction paradigm (+Chem: C6NYSA + T5J) or had 5-azaC or JNKin8 removed (5aza, JNKin8). Sample numbers of biologically independent cells: n = 8,826 (uninduced); 4,667 (+Chem, JNKin8); 10,257 (+Chem, 5aza); and 8,671 (+Chem stage II). Statistical comparisons are shown between groups by two-sided Wilcoxon test.