Scatter Plot from Scientific Research

Comparison of CRISPRi targeting in K562 cells engineered to constitutively express multiAsCas12a-KRAB (delivered by piggyBac) versus opAsCas12a (delivered by lentiviral transduction) using a subset of lentivirally transduced crRNA constructs from panel b, plus a crRNA construct targeting a coding exon of CD55 as a positive control for knockdown by DNA cutting. CD55 expression was assayed by flow cytometry 11 days after crRNA transduction. One-sided Wilcoxon rank-sum test was performed to compare the medians of single-cell expression knockdown of multiAsCas12a-KRAB versus opAsCas12a across n = 2–7 biological replicates (shown as individual data points, with vertical lines denoting standard error of the mean). *** P = 0.008; ** P = 0.033–0.036; * P = 0.05.

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