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K562 cells lentivirally transduced at MOI ~5 to constitutively express multiAsCas12a-KRAB were lentivirally transduced with single crRNAs targeting the HBG1/HBG2 TSS’s or their known enhancer, HS2. Shown are HBG1/HBG2 mRNA levels measured by RT-qPCR, normalized to GAPDH mRNA levels for two biological replicates shown as individual data points connected by vertical line to denote the range.
#Bar Plot#Error Bars#K562 Cells#Lentiviral Transduction#multiAsCas12a-KRAB#crRNAs#HBG1/HBG2 TSS#HS2 Enhancer#mRNA Levels#RT-qPCR#GAPDH#Biological Replicates
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