Library 1: Scatter plot of cell fitness scores in K562 cells for multiAsCas12a-KRAB versus denAsCas12a-KRAB for 3,357 single crRNA constructs with sufficient read coverage for analysis and targeting canonical TTTV PAMs within a -50-bp to +300-bp window of 559 essential gene TSSs. Marginal histograms show percentage of crRNA constructs with cell fitness score is lower than the 5th percentile of negative control crRNAs.
