Bar Plot from Scientific Research

Gene expression knockdown by multiAsCas12a-KRAB using 6-plex, 8-plex and 10-plex crRNA array constructs was measured by flow cytometry 10-11 days after lentiviral transduction of crRNA constructs. Shown are median gene expression knockdown averaged from two to four biological replicates, with error bars denoting standard error of the mean. From the two replicates for which all constructs were tested, one-sided Wilcoxon rank-sum test was performed to compare the single-cell distribution ( n > 200 cells for each replicate) of the 6-plex #1 construct against each of the other constructs; asterisks indicate P < 0.01 for both replicates.

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