Flow cytometry comparison of the indicated fusion protein constructs in dual CD55 and CD81 knockdown 10 days after lentiviral transduction of a 6-plex crRNA construct, shown for one biological replicate. Percentages of cells in each quadrant of the scatter plot, defined by the 5th percentile of non-targeting crRNA for each fluorescence signal, are indicated. One-sided, two-sample chi-square test was used to compare the proportion of cells ( n = 56–5,006 cells per replicate) with double knockdown between multiAsCas12a-KRAB versus each of the other fusion protein constructs; asterisks indicate P < 0.01.
