Comparison of CD81 knockdown by lentivirally delivered denAsCas12a-KRAB versus multiAsCas12a-KRAB at protein MOI ~1 versus ~5 across a panel of single and 3-plex crRNA constructs, maintaining a constant crRNA MOI within each paired comparison of denAsCas12a-KRAB versus multiAsCas12a-KRAB. Lines connect paired experiments within each biological replicate. crRNA MOI indicated by color scale. Dots indicate flow cytometry measurement 10 days after crRNA transduction; triangles indicate measurements 16 days after crRNA transduction.
