Line Plot from Scientific Research

Indel quantification from PCR amplicons surrounding target sites of crCD81-1 and crCD55-4 in cells lentivirally transduced at protein MOI ~5 for denAsCas12a-KRAB and multiAsCas12a-KRAB. Cells lentivirally transduced with opAsCas12a (DNase fully active) are shown for comparison. Percent of reads containing indels at each base position within the amplicon is plotted, with labels indicating maximum indel frequency observed across all bases within the amplicon. b – e , dashed lines indicate 5th percentile of measurements for non-targeting crRNA. Asterisks indicate P < 0.01 for one-sided Wilcoxon rank-sum test of single-cell distributions ( n > 200 cells per replicate) for a given paired comparison for all replicates shown.

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