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Schematic for assaying CRISPRi activity of AsCas12a constructs using lentivirally transduced single-plex or 3-plex crRNAs targeting cell surface marker genes assayed by antibody staining and flow cytometry.
#Workflow#Flowchart#Illustration#CRISPRi Activity#AsCas12a Constructs#Lentiviral Transduction#crRNAs#Cell Surface Marker Genes#Antibody Staining#Flow Cytometry
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