Scatter Plot from Scientific Research

log 2 FC z-score (IL2 + /NEG) for bidirectional perturbations, showing expected and observed. Expected log 2 FC z-score was calculated using additive model of single perturbations. Pairs with significant genetic interaction scores are highlighted (green; significance cutoffs are described in the Methods ). Each dot is an enhancer pair from one biological replicate. Data from IL2 locus validation screen, n = 3 (three biological replicates, 78 gRNAs per enhancer are aggregated). Box plot, median and interquartile range (IQR). Box whiskers, 1.5 IQR. Two-sided t-test. Significance was tested relative to NTC. Linear regression. Significance cutoffs: NS P > 0.05, * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001. exp, expanded; norm., normalized; NS, not significant; obs, observed.

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