Average log 2 FC z-score (IL2 + /NEG of all single, bidirectional and NTC gRNA pairs. Two gRNAs per enhancer (2 a, 2 i). RE hierarchy demonstrated when one perturbation overrides the expected effect of a second perturbation (for example, TSS.i bidirectional perturbations result in similar effect as TSS.i single perturbations; note that E6.i overrides other E.a). Results of specific columns and rows are expanded in subsequent figure panels.
