Histogram showing spike amplitude. n represents identified spike number. n = 53 for sLFM and n = 143 for csLFM. Format of all box plots: center line, median; box limits, lower and upper quartiles; and whiskers, 0th–100th percentiles excluding outliers. P values were all calculated by two-sided paired t -test, and P < 0.05 was considered statistically significant. Scale bars, 100 μm ( a , e ) and 50 μm ( h ). MIP, maximum intensity projection; STD, standard deviation; OCT, 3-octanol; MCH, 4-methylcyclohexanol; EA, ethyl acetate.