Comparison among sLFM, cLFM and csLFM in a mouse cortex labeled with GCaMP6f at 20 VPS. Displayed volumes are averages over time, and images are enlarged MIPs over the z axis. t = 0 s in each experiment represents the moment when the corresponding data started to be collected. Frames with similar neural responses are presented for comparison. The neuronal signals were captured at a center imaging depth of approximately 150 μm below the brain surface within an axial coverage of 60 μm. Scale bars, 100 μm.