Microscopy from Scientific Research

Orthogonal MIPs of the yellow box in b by cLFM (upper) and csLFM (lower) at different timestamps, showing the process of retraction fiber breakage and retractosome formation behind a migrating neutrophil. Only neutrophil channel is displayed in x - z images. White arrows point to some retractosomes, and gray arrows point to retraction fibers. Further enlarged regions of retractosomes are shown in the right. Normalized intensity profiles along the marked line before and after Gaussian fit are plotted with measured FWHM value.

DOI