Box plot showing SBR comparisons between sLFM and csLFM. The box plot format: center line, median; box limits, lower and upper quartiles; and whiskers, 0th–100th percentiles excluding outliers. P values were calculated by two-sided paired t -test, n = 12 cells. P = 9.62 × 10 −13 for dendritic cells and P = 2.77 × 10 −12 for T cells. P < 0.05 was considered statistically significant.