Graphical representation of the experimental design. Tryptic peptides from three species were combined in six distinct ratios (E5H50Y45, E10H50Y40, E20H50Y30, E30H50Y20, E40H50Y10 and E45H50Y5). Samples were processed using the Orbitrap Astral mass spectrometer in technical triplicates, employing a 3.5-ms maxIT and 2-Th window size method. The loading amounts were 200 ng and 800 ng.
