Schematic showing the experimental design to study the gene profiling of Allo15 BCAR-NKT cells using scRNA-seq. Conventional BCAR-T cells and non-IL-15 engineered Allo BCAR-NKT were included as two controls. Therapeutic cell samples were collected at three time points: day 0 (preinfusion samples), day 28 (in vivo samples) and day 35 (in vitro restimulated samples), followed by FACS and scRNA-seq analyses.
