Heatmap showing the log 2 fold change of frequency of nucleotide substitutions along the protospacer in predominantly blunt sites (blunt raw reads > 66%) compared to predominantly staggered sites (blunt raw reads < 33%) ( n = 26,802 sites with at least 16 reads in the PAM-proximal side). IGV, Integrative Genome Viewer; MM, mismatch; NT, non-target control.
