Box plots showing the average blunt rate for sites containing up to seven crRNA:DNA mismatches. n = 26,802 sites with at least 16 reads in the PAM-proximal side. Boxes characterize the sample using the lower quartile (Q1), median quartile (Q2) and upper quartile (Q3) and the interquartile range (IQR = Q3−Q1), and whiskers extend to the most extreme data point that is no more than 1.5× IQR from the edge of the box. The blue line depicts the best fit of a linear model relating blunt rate in target sites to mismatches (Pearson r = −0.26, P < 2.2 × 10 −16 ; n = 26,802 independent Cas9 on-targets/off-targets).
