– e are reproduced for the different loci (top to bottom: Gata4 , Foxa2 and Lamc1 ). Pseudobulk pileup of scATAC (pluripotent and parietal endoderm [Par. Endo]: Gata4 and Foxa2 , and also neuroectoderm and mesoderm for Lamc1 ) for 200 kb region centered on gene TSS. The gray shading of peaks indicate regions profiled (shaded red peak near Foxa2 TSS: peak not in the library due to inability to identify specific cloning primers). The carets point to elements identified as active with scQers. The inset for Lamc1 locus highlights differential accessibility in both pluripotent/epiblast and parietal endoderm cells.
